Relatedness Among Plants as Measured by the DNA-Agar Technique
- 1 July 1967
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 42 (7) , 959-967
- https://doi.org/10.1104/pp.42.7.959
Abstract
An improved method for extraction of plant DNA is described. Quantitative species comparisons based on DNA-DNA hybridization are reported for several members of the family Leguminosea and for barley, wheat and rye. A maximum of about 10% homology in DNA polynucleotide sequences is found between monocotyledons and dicotyledons tested, whereas 20 to 90% homology is observed within a family. Species compared using a DNA fraction enriched for redundant polynucleotide sequences generally appear to be more closely related than when whole DNA is used. DNA-DNA hybridization may be useful in systematic and evolutionary study of plants, and also as a possible screening procedure for interfertility of species.This publication has 11 references indexed in Scilit:
- Nucleotide Sequence Repetition: A Rapidly Reassociating Fraction of Mouse DNAScience, 1966
- Biochemical correlates of respiratory deficiency VI. Mitochondrial DNAJournal of Molecular Biology, 1966
- A quantitative assay for DNA-DNA hybrids using membrane filtersBiochemical and Biophysical Research Communications, 1966
- Nuclear Variation between Diploid AngiospermsNature, 1966
- A membrane-filter technique for the detection of complementary DNABiochemical and Biophysical Research Communications, 1966
- DNA associated with tobacco chloroplasts.Proceedings of the National Academy of Sciences, 1965
- A Molecular Approach in the Systematics of Higher OrganismsScience, 1964
- AN APPROACH TO THE MEASUREMENT OF GENETIC RELATEDNESS AMONG ORGANISMSProceedings of the National Academy of Sciences, 1963
- A GENERAL METHOD FOR THE ISOLATION OF RNA COMPLEMENTARY TO DNAProceedings of the National Academy of Sciences, 1962
- Thermal renaturation of deoxyribonucleic acidsJournal of Molecular Biology, 1961