Immobilization of plant protoplasts: Viability studies
- 1 February 1985
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 4 (1) , 23-27
- https://doi.org/10.1007/bf00285497
Abstract
Protoplasts of Daucus carota Ca68 and Catharanthus roseus have been immobilized by entrapment in gelforming polysaccharides (kappa-carrageenan, agarose and alginate). Uniform spherical beads of carrageenan and agarose containing the protoplasts have been prepared by utilizing an inert hydrophobic phase (vegetable oil). The entrapped protoplasts are viable and stabilized towards osmotic shock by the polymeric backbone. Standard methods have been used to study the viability and integrity of the entrapped protoplasts. Upon incubation in a relatively simple medium the immobilized protoplasts show a much higher viability after 14 days as compared to free protoplasts under the same conditions. The viability of D. carota protoplasts has also been monitored by an enzyme activity present in the cells (digitoxigenin 58-hydroxylase).Keywords
This publication has 11 references indexed in Scilit:
- Production of Biochemicals with Immobilized Plant Cells: Possibilities and ProblemsAnnals of the New York Academy of Sciences, 1983
- Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant speciesPlant Cell Reports, 1983
- A new procedure for increasing efficiency of protoplast plating and clone selectionPlant Cell Reports, 1983
- Permeabilization of immobilized plant cells, resulting in release of intracellularly stored products with preserved cell viabilityApplied Microbiology and Biotechnology, 1983
- A general method for the immobilization of cells with preserved viabilityApplied Microbiology and Biotechnology, 1983
- Improved protoplast culture and agarose mediaPlant Cell, Tissue and Organ Culture (PCTOC), 1983
- Immobilization of protoplasts by anchoring to microcarriersPlant Cell Reports, 1982
- Examination of parameters affecting the 5β-hydroxylation of digitoxigenin by immobilised cells of Daucus carotaApplied Microbiology and Biotechnology, 1981
- Immobilisation and mechanical support of individual protoplastsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1980
- Immobilized plant cells for the production and transportation of natural productsFEBS Letters, 1979