Transfection of murine LMTK? cells with purified HLA class I genes
- 1 April 1986
- journal article
- research article
- Published by Springer Nature in Immunogenetics
- Vol. 23 (4) , 246-251
- https://doi.org/10.1007/bf00373019
Abstract
The individual contributions of the first two external domains of the HLA-B7 heavy chain to the expression of allele-specific (B7) and locus-specific (B and C) antigenic determinants were investigated using hybrid class I genes. Hybrid genes were constructed in vitro by exon shuffling between the parent genes HLA-B7, HLA-Cw3, HLA-A3, and H-2Kd, and their expression was monitored following transfection into mouse L cells. The results show that most allele-specific antigenic determinants are associated with the first external domain of the 137 heavy chain, whereas all the locus-specific antigenic determinants tested map to the second external domain.Keywords
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