Electrophoretic Comparison of Polypeptides from Enriched Plasma Membrane Fractions from Developing Soybean Roots
- 1 December 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 66 (6) , 1037-1043
- https://doi.org/10.1104/pp.66.6.1037
Abstract
The polypeptide complement of enriched soybean (G. max (L.) Merr. cv. Wells) root plasma membrane fractions was studied by 2-dimensional gel electrophoresis. Good resolution was obtained when polypeptides were solubilized in sodium dodecyl sulfate and when butylated hydroxytoluene was included in the vesicle isolation and solubilization media. The pattern obtained on the 2-dimensional slab gel for root plasma membrane was characteristic for that membrane. The polypeptide complements from mitochondrial membranes and from enriched fractions of 3 other endomembrane components were solubilized and electrophoresed for comparison. Each membrane preparation was identifiable on the basis of its characteristic electrophoretogram. Electrophoresis of protein solubilized from plasma membrane fractions isolated from meristematic and mature root tissue revealed both qualitative and quantitative differences in the respective protein complements.This publication has 20 references indexed in Scilit:
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