IODINATION OF MONOCLONAL-ANTIBODIES FOR DIAGNOSIS AND RADIOTHERAPY USING A CONVENIENT ONE VIAL METHOD

  • 1 December 1986
    • journal article
    • research article
    • Vol. 27  (12) , 1890-1895
Abstract
We have developed a convenient system that can be used to iodinate monoclonal antibodies for diagnosis or therapy. A vial, previously coated with 1,3,4,6-tetrachloro-3a,6a-diphenyl glycouril (iodogen), used as a reaction vessel. Iodination and separation of bound and free iodide, using AG1-X8 ion exchange resin, are both accomplished in this vial. We found 90 .+-. 4% of the iodide which was added was incorporated, respectively, into each of four different monoclonal antibodies evaluated. Approximately 90% of labeled antibody was recovered in each case. The monoclonal antibody OC125 was labeled to specific activities up to 25 mCi/mg. Immunoreactivities of 82 .+-. 2% using 125I and 66 .+-. 5% using 131I were achieved. As the radioiodination is done in one sealed vial and takes < 15 min, this procedure is safe and can be performed in any nuclear medicine laboratory. The final product, which is sterile and apyrogenic, is suitable for diagnostic and radiotherapeutic applications.