DNA sequence of the araBAD promoter in Escherichia coli B/r.

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Abstract
The L-arabinose operon in E. coli is a model system for the study of the control of gene expression. Maximal expression of the araBAD operon requires 2 positive control components: the araC protein-L-arabinose complex and the cyclic[c]AMP receptor protein-cAMP complex. Both araC protein and cAMP receptor protein are required for the initiation of transcription of araBAD mRNA. Plasmid pBR322 was used as a vector for cloning DNA fragments that contain the araBAD promoter. The cloned ara fragments were identified by both physical and genetic tests. A restriction map was constructed and the DNA sequence of the promoter was determined. The promoter contains a site that is similar to the RNA polymerase recognition sites in the galactose and lactose operons. It also contains a region similar to the known cAMP receptor protein binding sites in the galactose and lactose operons.