ANTIGEN-SPECIFIC AND NONSPECIFIC MEDIATORS OF T-CELL-B-CELL COOPERATION .4. DEVELOPMENT OF A MODEL SYSTEM DEMONSTRATING RESPONSIVENESS OF 2 T-CELL FUNCTIONS TO HGG INVITRO

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 117  (5) , 1953-1959
Abstract
Previous work in this laboratory defined 2 functionally distinct T [thymus-derived] cell subpopulations (antigen-specific helper and antigen-nonspecific helper) and characterized some of the parameters of these subpopulations derived from the keyhole limpet hemocyanin (KLH)-primed mouse spleen. Characterization of further parameters such as relative susceptibility to tolerance induction and relative degree of specificity was not possible with the use of KLH as the antigen. To overcome this impediment, an experimental protocol was developed to permit the study of these 2 T cell subpopulations in response to the antigen human .gamma.-globulin (HGG). This protocol qualitatively duplicates the parameters defined for the KLH system and provides an extremely useful model for the study of the response to serum proteins in the Mishell-Dutton culture system.