MACROPHAGE COLONY FORMATION SUPPORTED BY PURIFIED CSF-1 AND OR INTERLEUKIN-3 IN SERUM-FREE CULTURE - EVIDENCE FOR HIERARCHICAL DIFFERENCE IN MACROPHAGE COLONY-FORMING CELLS

  • 1 April 1986
    • journal article
    • research article
    • Vol. 67  (4) , 859-864
Abstract
Using a serum-free culture system, we examined murine macrophage colony formation from bone marrow cells cultured in the presence of purified CSF-1, interleukin 3 (IL 3) or a combination of the two factors. CSF-1 supported macrophage and neutrophil-macrophage colony formation, whereas IL-3 supported the formation of various types of single lineage and multilineage colonies. CSF-1 supported more macrophage colonies from bone marrow cells of normal mice than IL 3, whereas in cultures of bone marrow cells of 5-fluorouracil-treated mice, IL 3 supported more macrophage colonies. A combination of CSF-1 and IL 3 resulted in granulocyte-macrophage (GM) colony formation that was equal to or greater than the sum of GM colony formation supported by the factors individually. The combination of CSF-1 and IL 3 resulted in significant increases in the size of both macrophage and neutrophil-macrophage colonies. Similar increases in colony size were observed when CSF-1 was added to cultures five days after incubation of marrow cells with IL 3. These data support the concept that some of the macrophage colony-forming cells that respond to IL 3 are more primitive than those that are sensitive to CSF-1.

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