Fourteen polymorphic microsatellite markers for the fungal banana pathogen Mycosphaerella fijiensis
- 28 June 2008
- journal article
- Published by Wiley in Molecular Ecology Resources
- Vol. 8 (4) , 910-912
- https://doi.org/10.1111/j.1755-0998.2008.02113.x
Abstract
Fourteen polymorphic microsatellite markers were developed for Mycosphaerella fijiensis, a fungus causing the black sigatoka disease in banana. The sequenced genome of M. fijiensis was screened for sequences with single sequence repeats (SSRs) using a Perl script. Fourteen SSR loci, evaluated on 48 M. fijiensis isolates from Hawaii, were identified to be highly polymorphic. These markers revealed two to 19 alleles, with an average of 6.43 alleles per locus. The estimated gene diversity ranged from 0.091 to 0.930 across the 14 microsatellite loci. The SSR markers developed would be useful for population genetics studies of M. fijiensis.Keywords
This publication has 15 references indexed in Scilit:
- Challenges of microsatellite isolation in fungiFungal Genetics and Biology, 2007
- Identification and genetic mapping of highly polymorphic microsatellite loci from an EST database of the septoria tritici blotch pathogen Mycosphaerella graminicolaFungal Genetics and Biology, 2007
- Development of VNTR markers for two Fusarium graminearum clade speciesMolecular Ecology Notes, 2004
- Genetic structure of Mycosphaerella fijiensis populations from Australia, Papua New Guinea and the Pacific IslandsPlant Pathology, 2003
- Characterization of nine polymorphic microsatellite loci in the fungus Botrytis cinerea (Ascomycota)Molecular Ecology Notes, 2002
- An economic method for the fluorescent labeling of PCR fragmentsNature Biotechnology, 2000
- Oligonucleotide Fingerprinting Detects Genetic Variability at Different Levels in Nigerian Mycosphaerella fijiensisJournal of Phytopathology, 1997
- Genetic structure of the global population of banana black leaf streak fungus, Mycosphaerella fijiensisMolecular Ecology, 1996
- DNA Restriction Fragment Length Polymorphisms inMycosphaerellaSpecies That Cause Banana Leaf Spot DiseasesPhytopathology®, 1994
- Analysis of Gene Diversity in Subdivided PopulationsProceedings of the National Academy of Sciences, 1973