A Rapid, Simple, and Reliable Technique for Preparation of Antisera against Idiotypes of Homogeneous Immunoglobulins
- 9 January 1978
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 35 (1-2) , 10-12
- https://doi.org/10.1111/j.1423-0410.1978.tb02894.x
Abstract
One-step immunization of guinea pigs with homogeneous immunoglobulins [Ig] partly purified by micro-agar electrophoresis represents a rapid and reliable microtechnique for the preparation of antisera against the idiotype of the homogeneous Ig. Testing of these antisera by immunodiffusion techniques requires agar plates containing 3% polyethylene glycol 6000.This publication has 4 references indexed in Scilit:
- Detection of human IgA subclasses and allotypes by polymer-induced precipitation in agarose gelJournal of Immunological Methods, 1974
- Homogeneous Immunoglobulins in Sera of Mice During AgingThe Journal of Immunology, 1974
- The Production of Anti-Idiotypic Antibodies to BALB/c Plasmacytoma Globulins in BALB/c MiceThe Journal of Immunology, 1972
- Polymer-induced precipitation of antigen-antibody complexes: ‘Precipiplex’ reactionsImmunochemistry, 1971