Enrichment of denitrifying glycogen‐accumulating organisms in anaerobic/anoxic activated sludge system

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Abstract
Denitrifying glycogen‐accumulating organisms (DGAO) were successfully enriched in a lab‐scale sequencing batch reactor (SBR) running with anaerobic/anoxic cycles and acetate feeding during the anaerobic period. Acetate was completely taken up anaerobically, which was accompanied by the consumption of glycogen and the production of poly‐β‐hydroxy‐alkanoates (PHA). In the subsequent anoxic stage, nitrate or nitrite was utilized as electron acceptor for the oxidation of PHA, resulting in glycogen replenishment and cell growth. The above phenotype showed by the enrichment culture demonstrates the existence of DGAO. Further, it was found that the anaerobic behavior of DGAO could be predicted well by the anaerobic GAO model of Filipe et al. (2001) and Zeng et al. (2002a). The final product of denitrification during anoxic stage was mainly nitrous oxide (N2O) rather than N2. The data strongly suggests that N2O production may be caused by the inhibition of nitrous oxide reductase by an elevated level of nitrite accumulated during denitrification. The existence of these organisms is a concern in biological nutrient removal systems that typically have an anaerobic/anoxic/aerobic reactor sequence since they are potential competitors to the polyphosphate‐accumulating organisms. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 81: 397–404, 2003.