Storage of 5‐Hydroxytryptamine in Rat Mast Cells. Evidence for an Ionic Binding to Carboxyl Groups in a Granule Heparin‐Protein Complex

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Abstract
Bergendorff, A. and B. Uvnas.Storage of 5‐hydroxytryptamine in rat mast cells. Evidence for an ionic binding to carboxyl groups in a granule heparin‐protein complex.Acta physiol. scand. 1972.84.320–331.Basophil granules were isolated from rat peritoneal and thoracic mast cells. The granules were depleted of their endogenous Hi and 5‐HT and the amine storing properties of these depleted granules were studied. The influence of pH, the uptake capacity, the releasing action of sodium ions and the competitive uptake of Hi and 5‐HT by the granule stores all support the hypothesis that the granule stores have the properties of a weak cation exchange resin. It appears that 5‐HT release, as we previously showed for Hi‐release, is due to a cation exchange occurring on mast cell degranulation, when the amine‐containing granules become exposed to cations of the extracellular milieu.