Influence of Oxygen on the Induction of Mutations in Bacteriophage ΦX174 by Ionizing Radiation
- 1 January 1974
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine
- Vol. 25 (5) , 505-512
- https://doi.org/10.1080/09553007414550671
Abstract
Several mutants of bacteriophage ΦX174 having nonsense codons in different cistrons are reverted with a higher efficiency to pseudo wild-type particles, i.e. particles able to propagate in suppressorless host bacteria, when irradiated with γ-rays in the presence of oxygen than when irradiated under anoxic conditions. For an ochre mutant of ΦX174 it was found that the radiation-induced conversion into an amber mutant, which corresponds with a transition of adenine in the parental DNA into guanine in the progeny DNA, is enhanced by oxygen. Differences between amber mutants of more than two-hundred fold in the efficiency of induction of pseudo wild-types show that the local environment of the amber codon in the phage particle exerts a great influence on the induction.Keywords
This publication has 8 references indexed in Scilit:
- Two Proteins of Gene A of ϕX174Nature New Biology, 1973
- Reversion of cistron A amber mutants of bacteriophage ϕ 164 by ionizing radiationMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1973
- Genetic Map of Bacteriophage φX174Journal of Virology, 1971
- Mutagenic Methods for determining which DNA Strand is transcribed for Individual Viral GenesNature, 1970
- Sense and Nonsense in the Genetic CodeScience, 1968
- The process of infection with bacteriophage ΦX174Journal of Molecular Biology, 1966
- Identification of the altered bases in mutated single-stranded DNAJournal of Molecular Biology, 1964
- DNA CIRCULARITY AND THE MECHANISM OF STRAND SELECTION IN THE GENERATION OF GENETIC MESSAGESProceedings of the National Academy of Sciences, 1964