Transformation of human cells by DNAs ineffective in transformation of NIH 3T3 cells.

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Abstract
Neonatal human foreskin fibroblasts can be transformed to anchorage-independent growth by transfection with DNA-inefficient in transforming [mouse] NIH 3T3 cells. Human cells transfected with DNA from GM 1312, a [human] multiple myeloma cell line, or MOLT-4, a permanent [human] lymphoblast line, grown without anchorage at a much higher frequency than do the parental cells and their DNA can transform human cell recipients to anchorage-independent growth; they have extended but not indefinite life spans and are nontumorigenic. Human fibroblasts are also transformed by DNA from 2 multiple myeloma lines that also transform 3T3 cells; restriction analysis suggests that different transforming genes in this DNA are acting in the human and murine systems. The human cell transfection system allows detection of transforming genes not effective in the 3T3 system and points out the possibility of detection of additional transforming sequences even in DNA that do transform murine cells. [This study has relevance for oncogenesis].