PHOSPHORUS METABOLISM OF THE ADRENAL GLAND: THE MECHANISM OF THE CHANGE AFTER HYPOPHYSECTOMY

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Abstract
The specific activity of intracellular inorganic phosphate (P) of the adrenal gland relative to that of the inorganic P of the plasma is significantly decreased in hypophysectomized rats at time intervals as long as 16 hrs. after injn. of inorganic P labelled with p32. The specific activity of the intracellular inorganic P was detd. (1) by measuring the specific activity of the easily -hydrolyzable acid-soluble P, which rapidly comes into isotope equilibrium with the intracellular inorganic P, (2) by calculation, from the specific activity of the inorganic P of the whole adrenal, assuming values for the specific activity and the concn. of the inorganic P of the extracellular fluid and the volume of the extracellular fluid compartment, and (3) by measuring the specific activities of the inorganic P of both the adrenal gland and the plasma at a series of time intervals after injn.of p32. It is concluded that decrease in relative specific activity of the intracellular inorganic P of the adrenal is the result of a slowing in the passage of inorganic p32 across the cell membrane, i.e., from the extracellular to the intracellular fluid.