Antibodies against Human Leukemia Cell Lines

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Abstract
Summary: Five groups of antibodies differing in their specificities were characterized in rabbit antisera against two cultured human leukemia cell lines, #6410 and A-LK1D. These are: 1) antibodies specific to individual leukemia cell lines, 2) antibodies reacting with other leukemia cell lines as well, 3) antibodies reacting as in 2 and in addition with lymphoid organs and with leukocytes, 4) antibodies reacting as in 3 and in addition with liver and kidney and 5) antibodies reacting as in 4 and in addition with nonleukemia cell lines of human origin. The groups were characterized by the use of a paired label radio-antibody technique adapted to tissue sections or cell smears to test the reactivities of remaining antibodies after exhaustive absorption of antibodies with various organs and cultured cells.