Characterization and primary structures of DNA‐binding HU‐type proteins from Rhizobiaceae
- 3 March 1985
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 147 (2) , 343-349
- https://doi.org/10.1111/j.1432-1033.1985.tb08755.x
Abstract
The DNA‐binding HU‐type proteins from several species of Rhizobiaceae including Rhizobium meliloti, two strains of Rhizobium leguminosarum with highly different phenotypic characters and Agrobacterium tumefaciens, were characterized and their amino acid sequences were determined. HU‐type proteins isolated from R. leguminosarum L18 and A. tumefaciens are identical and show slight differences with the R. meliloti HU‐type protein. On the other hand the R. leguminosarum L53 HU‐type protein is quite different from the proteins cited above; several amino acid substitutions encountered in this protein result in significant changes in the folding of the polypeptide chain. The biochemical characteristics of these proteins are in good agreement with the respective position of these bacteria in the phylogeny determined by numerical taxonomy.This publication has 24 references indexed in Scilit:
- Comparative study of the DNA-binding HU-type proteins from slow growing and fast growing strains of RhizobiaceaeBiochemical and Biophysical Research Communications, 1984
- Proteins from the prokaryotic nucleoidFEBS Letters, 1984
- Characterization and structural study of the DNA-binding protein HRm from Rhizobium melilotiBiochemical and Biophysical Research Communications, 1982
- Purification and Characterisation of a Small DNA‐Binding Protein, HB, from Bacillus globigiiEuropean Journal of Biochemistry, 1982
- A single DNA‐binding protein from Pseudomonas aeruginosa homologous to proteins NS1 and NS2 (HU proteins) of Escherichia coli and other bacteriaFEBS Letters, 1981
- Purification of B.subtilis DNA-binding protein related to E.coli HUBiochemical and Biophysical Research Communications, 1980
- Complete Amino‐Acid Sequences of DNA‐Binding Proteins HU‐1 and HU‐2 from Escherichia coliEuropean Journal of Biochemistry, 1980
- Primary structures of two homologous ribosome‐associated DNA‐binding proteins of Escherichia coliFEBS Letters, 1978
- Direct microsequence analysis of polypeptides using an improved sequenator, a nonprotein carrier (Polybrene), and high pressure liquid chromatographyBiochemistry, 1978
- The amino‐ and carboxy‐terminal amino acid sequences of protein HU from Escherichia coliFEBS Letters, 1978