Characterization of lactate–guanidinium and lactate–lactate interactions in aqueous solution by spectropolarimetry
- 1 January 1996
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Perkin Transactions 2
- No. 7,p. 1419-1422
- https://doi.org/10.1039/p29960001419
Abstract
Spectropolarimetry has been used to quantify the lactate–guanidinium and lactate–lactate equilibrium interactions in aqueous solution. Association constants for the lactate–guanidinium and lactate–lactate formations are 6.11 dm3 mol–1 and 1.12 dm3 mol–1, respectively, in aqueous solution; 6.11 dm3 mol–1 is a high value for an electrostatic interaction in water. This stability, however, cannot account for the extremely strong lactate–protein binding, observed earlier by NMR spectroscopy. The molar rotation coefficients for both the heteroassociation and homoassociation complexes are also calculated. The homoassociative lactate–lactate binding is the first such interaction, whose constant has been determined by spectropolarimetry in aqueous solution.Keywords
This publication has 13 references indexed in Scilit:
- Selective binding and sensing of guanidinium ions by lipophilic cyclodextrinsJournal of the Chemical Society, Perkin Transactions 2, 1994
- 1H NMR studies on the preferred interactions of guanidinium and C-terminal carboxylate groups in arginine-containing peptidesJournal of the Chemical Society, Perkin Transactions 2, 1993
- Microscopic acid—base equilibria of arginineTalanta, 1991
- Enantiodifferentiation of carboxylates by chiral building blocks for abiotic anion receptorsJournal of the Chemical Society, Chemical Communications, 1990
- A review of the application of chiroptical methods to analytical chemistryJournal of Pharmaceutical and Biomedical Analysis, 1989
- NMR‐invisible lactate in blood plasmaFEBS Letters, 1988
- Lactic acidosis: a metabolic complication of extensive metastatic cancerEuropean Journal of Cancer and Clinical Oncology, 1983
- A unified view of carbon bound hydrogen exchange of H(2) in imidazoles and H(8) in purine nucleosides and their metal ion complexesJournal of the American Chemical Society, 1982
- Structure of the active ternary complex of pig heart lactate dehydrogenase with S-lac-NAD at 2.7 Å resolutionJournal of Molecular Biology, 1981
- LACTIC ACIDAnnals of the New York Academy of Sciences, 1965