TRIGGERING OF MONOCLONAL HUMAN LYMPHOMA B-CELLS WITH ANTIBODIES TO IGM HEAVY-CHAINS - DIFFERENCES OF RESPONSE OBTAINED WITH MONOCLONAL AS COMPARED TO POLYCLONAL ANTIBODIES

  • 1 January 1983
    • journal article
    • research article
    • Vol. 54  (3) , 756-764
Abstract
A comparative study of human B lymphoma cells activation by monoclonal (murine hybridoma) antibodies to .mu. H chains (Ma-.mu.), compared to polyclonal (rabbit) antibodies to .mu. H chains (Ra-.mu.) was carried out. Early events related to calmodulin activation such as 86Rb influx and changes in cell volume at 4 h could be induced by Ma-.mu.. One antibody (AF6) approached Ra-.mu. with regard to the strength of response obtained. Ma-.mu.s including AF6 were deficient in inducing DNA synthesis under conditions where this was achieved with Ra-.mu.. Studies in 1 lymphoma, where stimulation of re-expressed surface IgM [sIgM] could be studied, revealed that Ma-.mu. was deficient in stimulating re-expressed sIgM. The findings raise questions with regard to polyclonal antibody to surface Ig as a model for B cell triggering by antigen and suggest that antigen-induced B cell triggering may be more complex than indicated by previous studies with polyclonal antibody.