RelAmutation and pBR322 plasmid amplification in amino acid-starved cells ofEscherichia coli
- 1 December 1989
- journal article
- research article
- Published by Hindawi Limited in Genetics Research
- Vol. 54 (3) , 167-171
- https://doi.org/10.1017/s0016672300028627
Abstract
Summary: Plasmid pBR322 is amplified following amino-acid limitation inEscherichia coli relAhosts. InrelA+hosts there was no significant amplification or a much smaller one. Plasmid amplification is due to therelAmutation; when therelA+allele is transferred into therelAmutant CP79 this strain no longer amplifies plasmid DNA during amino acid starvation. It is concluded that ppGpp is a negative effector of plasmid replication. Amplification is temperature dependent, being maximal at 32 °C and negligible at 37 °C.This publication has 18 references indexed in Scilit:
- Expression of a cloned β-glucanase gene from Bacillus amyloliquefaciens in an Escherichia coli relA strain after plasmid amplificationMolecular Genetics and Genomics, 1988
- Differential inhibition of the initiation of DNA replication in stringent and relaxed strains ofEscherichia coliGenetics Research, 1988
- Analysis of the physiological control of replication of ColE1-type plasmidsJournal of Theoretical Biology, 1986
- Increased Amplification of Plasmids pBR322 and pBR327 by Low Concentrations of ChloramphenicolDNA, 1986
- Escherichia coli relA strains as hosts for amplification of pBR322 plasmid DNAFEMS Microbiology Letters, 1985
- Regulation of plasmid copy number by complementary RNAsTrends in Biochemical Sciences, 1985
- Mechanism of control of DNA replication and incompatibility in ColE1-type plasmids —a reviewGene, 1984
- A relaxed mutant with an altered ribosomal protein L11Molecular Genetics and Genomics, 1976
- A New Relaxed Mutant of Escherichia coli with an Altered 50S Ribosomal SubunitProceedings of the National Academy of Sciences, 1974
- An improved method for thin-layer chromatography of nucleotide mixtures containing32P-labeled orthophosphateJournal of Chromatography A, 1969