Actin depolymerization in the cyclic AMP‐stimulated toad bladder epithelial cell, determined by the DNAse method

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Abstract
Previous studies with the rhodamine phalloidin binding assay have shown that antidiuretic hormone and 8‐Br‐cAMP rapidly depolymerize F‐actin in toad bladder epithelial cells. We have extended these studies with DNAse inhibition assay and have found that in isolated epithelial cell suspensions, G‐actin increases from 37 to 56% of total actin following 8‐br‐cAMP stimulation. The G‐actin concentration in the epithelial cell greatly exceeds its critical concentration, indicating the requirement for a G‐actin sequestering protein or proteins in this system.