Extracellular enzyme activities of dermatophytes and yeast isolates on solid media
- 1 December 1997
- Vol. 40 (11-12) , 465-469
- https://doi.org/10.1111/j.1439-0507.1997.tb00186.x
Abstract
Summary. A total of 123 isolates of 14 species of dermatophytes and yeasts were screened for the activity of five extracellular enzymes including elastase, keratinase, protease (gelatinase), lipase and phospholipase, by using solid media. The optimal production and activity of each enzyme was determined with regard to different pH and temperatures. Keratinase activity was high with all the tested fungi with exception of Malassezia furfur. Protease (gelatinase) was produced only by dermatophytes. Elastase was secreted by three dermatophytes viz. Microsporum gypseum, Trichophyton mentagrophytes var. mentagrophytes and T. verrucosum, wheieas lipase and phospholipase were detected in all the species except T. violaceum. Zusammenfassung. Die Aktivität von fünf extrazellulären Enzymen (Elastase, Kératinase, Protease, Lipase und Phospholipase) wurde auf festen Medien an 123 Isolaten von 14 Dermatophyten- und Hefe-Arten untersucht. Es wurden die Aktivitätsoptima in Abhängigkeit von pH-Wert und Temperatur bestimmt. Die Keratinase-Aktivität war bei allen geprüften Pilzen mit Ausnahme von Malassezia furfur hoch. Protease-Aktivität zeigten nur die Dermatophyten. Elastase-Aktivität war bei drei Dermatophyten, Microsporum gypseum, Trichophyton mentagrophytes var. mentagrophytes und T. verrucosum zu beobachten. Lipase- und Phospholipase-Aktivität zeigte sich bei allen geprüften Pilzarten außer T. violaceum.Keywords
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