Effects of Extended Low-dose-rate Exposure to137Cs Detected by Flow-cytometric Enumeration of Micronucleated Erythrocytes in Mouse Peripheral Blood
- 1 January 1993
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 63 (3) , 339-347
- https://doi.org/10.1080/09553009314550451
Abstract
An automated variant the of micronucleus assay for erythrocytes in mouse peripheral blood has recently been developed. The flow-cytometric technique used allows very large numbers of erythrocytes to be analysed with a relatively small effort. Here we report the potential of this method to detect a response to extended low-dose-rate exposure to γ-irradiation. The mice were irradiated with a 137Cs source at a dose rate of 4·8 cGy/day for 26 days. Sampling was continued for another 39 days after irradiation. Elevated frequencies compared with the control group were found at days 2, 9 and 20 after the start of the irradiation for micronucleated polychromatic erythrocytes, and at days 9, 20, 29, 42, 51 and 65 for micronucleated normochromatic erythrocytes.Keywords
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