Human platelet antigen4 (Br) genotyping by ASPA: allele‐specific primer amplification (PCR‐SSP)
- 1 November 1994
- journal article
- Published by Wiley in British Journal of Haematology
- Vol. 88 (3) , 659-661
- https://doi.org/10.1111/j.1365-2141.1994.tb05096.x
Abstract
We have developed a PCR assay named ASPA (allele-specific primer amplification) to determine the HPA-5a and -5b genotypes. It consists of two PCR-reactions. One primer of each primer set has a 3'-end nucleotide which is specific for A or G at position 505 of the HPA-5b or -5a allele respectively. The HPA-5 genotypes determined in this way were strictly concordant with the genotypes established by the PCR-ASRA and with the phenotypes established using MAIPA. The ASPA is a rapid and reliable technique and can be used for the determination of alleles which code for platelet antigen allotypes.Keywords
This publication has 9 references indexed in Scilit:
- The human platelet alloantigens, HPA-5(a+ b-) and HPA-5(a- b+), are associated with a Glu505/Lys505polymorphism of glycoprotein Ia (the α2subunit of VLA-2)British Journal of Haematology, 1994
- Localization of the Br Polymorphism on a 144 bp Exon of the GPIa Gene and Its Application in Platelet DNA TypingThrombosis and Haemostasis, 1994
- The human platelet alloantigens Br(a) and Brb are associated with a single amino acid polymorphism on glycoprotein Ia (integrin subunit alpha 2).Journal of Clinical Investigation, 1993
- HPA‐1 typing by PCR amplification with sequence‐specific primers (PCR‐SSP): a rapid and simple techniqueBritish Journal of Haematology, 1993
- Prenatal diagnosis of neonatal alloimmune thrombocytopenia using allele- specific oligonucleotide probesBlood, 1991
- A simple method for DNA purification from peripheral bloodAnalytical Biochemistry, 1988
- Isolation and characterization of a platelet surface collagen binding complex related to VLA-2Biochemical and Biophysical Research Communications, 1988
- Monoclonal antibody--specific immobilization of platelet antigens (MAIPA): a new tool for the identification of platelet-reactive antibodiesBlood, 1987
- Integrins: A family of cell surface receptorsCell, 1987