A New Phosphorylating Agent, Di(2,2,2-Trifluoroethyl) Trimethylsilyl Phosphite. Its Application in Dna Synthesis by The Phosphotriester Approach
- 1 December 1985
- journal article
- research article
- Published by Taylor & Francis in Nucleosides, Nucleotides and Nucleic Acids
- Vol. 4 (5) , 669-679
- https://doi.org/10.1080/07328318508081899
Abstract
A new phosphorylating agent, di(2,2,2-trifluoro-ethyl) trimethylsilyl phosphite, has been prepared and is proved to be a useful agent for the phosphorylation of the 3′-hydroxyl group of deoxyribonucleosides in the absence of coupling agents. The resulting deoxyribonucleoside 3′-(2,2,2-trifluoroethyl) phosphates are key intermediates for the synthesis of deoxyribooligonucleotides by the phospho-triester approach.This publication has 13 references indexed in Scilit:
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