A stereochemical study of the mechanism of activation of donor oligonucleotides by RNA ligase from bacteriophage T4 infected Escherichia coli
- 1 December 1985
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 24 (25) , 7446-7449
- https://doi.org/10.1021/bi00346a062
Abstract
RNA ligase from bacteriophage T4 infected Escherichia coli catalyzes the activation of adenosine 3'',5''-bisphosphate (representing the donor oligonucleotide) by adenosine 5''-[(S)-.alpha.-17O,.alpha.,.alpha.-18O2]triphosphate with retention of configuration at P.alpha.. Since single-step enzyme-catalyzed nucleotidyl transfer reactions proceed with inversion, this stereochemical result provides support for a double displacement mechanism involving an adenylyl-enzyme intermediate as proposed previously from isotope exchange experiments.Keywords
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