Enzyme‐aided construction of medium‐sized alditols of complete O‐linked saccharides
- 25 July 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 349 (1) , 55-59
- https://doi.org/10.1016/0014-5793(94)00638-5
Abstract
We have constructed by enzyme-aided in vitro synthesis a hexasaccharide alditol Galβ1—4GlcNAcβ1—6Galβ1—4GlcNAcβ1—6(Galβ1—3)GalNAc-ol and shown that it resists the action of endo-β-galactosidase from Bacteroides fragilis under conditions where a related pentasaccharide alditol, GlcNAcβ1—3Galβ1—4GlcNAcβ1—6(Galβ1—3)GalNAc-ol, was completely cleaved. Together with earlier results from this laboratory, our present data imply that endo-β-galactosidase from B. fragilis, apparently, can be used to distinguish between GlcNAcβ1—6Gal and GlcNAcβ1—3Gal units within linear backbone sequences of all known types of oligo-(N-acetyllactosamino)glycans.Keywords
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