Evaluation of Male Infertility by Penetrability (or Penetration Assay) of the Human Spermatozoa through Zona‐Free Hamster Ova

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Abstract
The human spermatozoa zona‐free hamster ova penetration assay (SPA) and seminal fluid analysis (SFA) were performed on 44 infertile and 35 fertile couples. The mean penetration rate for semen samples from fertile males (44% ± 3%) was significantly higher (p0.05) was observed between the spermatozoal density, total spermatozoal count, percentage of motile spermatozoa, or percentage of normal morphology and the penetration rate. The sensitivity of the SPA was 91% and the specificity was 100% as compared to 73% and 83% respectively for the SFA. These findings indicate that the SPA is useful in the assessment of the fertility potential of human semen samples in the evaluation of male fertility and infertility.