Identification of a Sperm Receptor-Glycoprotein from the Porcine Zona pellucida

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Abstract
Porcine zona pellucida were solubilized by treatment with lithium 3,5-diiodosalicylate. Following further purification by phenol/water extraction, ethanol precipitation and high performance liquid chromatography a total of 11 fractions were obtained. Fractions in the molecular mass range that were previously found to possess antigenic properties were tested in a spermatozoa radioreceptor binding assay. One of the fractions with an N-terminal amino-acid residue of alanine had spermatozoa receptor properties. This result might aid future studies on the spermatozoa-zona pellucida interactions at the molecular level.