A Simple Method for Preparation of Highly Purified Vasopressin.
- 1 December 1957
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 96 (3) , 568-570
- https://doi.org/10.3181/00379727-96-23542
Abstract
Both arginine- and lysine-vasopressin were obtained in a high degree of purity by means of chromatography on carboxymethyl cellulose ion exchangers using ammonium acetate as a volatile buffer and differential elution gradients. Starting material for the chromatog-raphy had a potency of from 22 to 35 pressor units per mg and 10 to 12-fold purification was obtained in a single passage over the ion exchange column. Conditions for the resolution of arginine- and lysine-vasopressin with this chromatography were described. High flow rates, efficient recovery of activity, and rapid removal of buffers by lyophiliza-tion provided a simple means for purifying vasopressin.Keywords
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