Horizontal gradient polyacrylamide gel electrophoresis

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Abstract
A simple Technique for horizontal gradient polyacrylamide gel electrophoresis (PAGE) in discontinuous buffer systems is described, in which one‐dimensional, two‐dimensional and double one‐dimensional separation patterns can be obtained. The advantages of this technique are: (i) that samples can be applied by overlaying on a variable broad area of the concentrating gel, (ii) that the complete separating gel and the contact with the concentrating gel remain sandwiched between glass plates from the moment of gel polymerization till the end of electrophoretic separation, (iii) that many gels are poured from one gel solution, and (iv) that the gels can be stored, due to an identical buffer composition in the concentrating and separating gel.