A 5' to 3' exonuclease functionally interacts with calf DNA polymerase epsilon.
- 15 October 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (20) , 9377-9381
- https://doi.org/10.1073/pnas.89.20.9377
Abstract
Analysis of fractions containing purified DNA polymerase epsilon from calf thymus has revealed the presence of a 5' to 3' exonuclease activity that is specific for a single strand of duplex DNA. This activity is capable of degrading a 3'-labeled oligonucleotide hybridized to M13mp18 DNA. When a second oligonucleotide primer is annealed 3 bases upstream, degradation of the downstream primer is strictly dependent on DNA synthesis from the upstream primer. Replacement of the downstream primer by an oligoribonucleotide of identical sequence results in a similar pattern of exonucleolytic activity. The activity has been highly purified and found to cosediment in glycerol gradients with a peptide of 56 kDa as judged by SDS/PAGE analysis. Effects of calf DNA polymerase alpha and delta on exonuclease activity are also observed but with differences in the pattern of products.Keywords
This publication has 24 references indexed in Scilit:
- Structural relationships between two forms of DNA polymerase epsilon from calf thymus.Journal of Biological Chemistry, 1992
- Synthesis of DNA by DNA polymerase epsilon in vitro.Journal of Biological Chemistry, 1991
- Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.Proceedings of the National Academy of Sciences, 1991
- Influence of poly(ADP-ribose) polymerase on the enzymatic synthesis of SV40 DNA.Journal of Biological Chemistry, 1991
- Properties of DNA polymerases δ and ϵ, and their roles in eukaryotic DNA replicationBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1991
- Discontinuous DNA synthesis by purified mammalian proteins.Journal of Biological Chemistry, 1990
- SUBSTRATE-SPECIFICITY OF THE EXONUCLEASE ASSOCIATED WITH CALF DNA POLYMERASE-EPSILON1990
- A third essential DNA polymerase in S. cerevisiaeCell, 1990
- Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication originNature, 1990
- An active fragment of DNA polymerase produced by proteolytic cleavageBiochemical and Biophysical Research Communications, 1969