Photoconversion in orange and red fluorescent proteins

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Abstract
Several red and orange fluorescent proteins are reported to be photoconvertible. Specifically, three red fluorescent proteins that can be switched to green, and two orange fluorescent proteins that can be switched to far red are reported. We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date.