The Release of Auxin from Isolated Leaf Proteins of Spinach by Enzymes

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Abstract
Auxin associated with proteins isolated from the leaves of spinach can be released by enzymatic hydrolysis; auxin is obtained from both cytoplasmic and chloroplastic proteins. Cytoplasmic proteins can be separated into 2 fractions by isoelectric precipitation; more auxin is obtained from one of these fractions than from the other. Diffusion expts. indicate similarity between leaf auxin and the auxin released from proteins by enzymatic hydrolysis. Both auxins appear of lower mol. wt. than indole acetic acid. The auxin released from leaf proteins of spinach is not a result of bacterial contamination.