Aberrant Migration of Lipopolysaccharide in Sodium Dodecyl Sulfate/Poyacrylamide Gel Electrophoresis

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Abstract
Purified lipopolysaccharides of salmonellae strains were separated by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Pre-electrophoresis of polyacrylamide gels had no apparent effect on 1-dimensional Ag-stained lipopolysaccharide profiles. Without pre-electrophoresis, 2-dimensional and 3-dimensional patterns contained numerous bands with varied migration patterns compared to those in the 1-dimension gels. The lipopolysaccharide was altered within the polyacrylamide gel during electrophoresis. Pre-electrophoresis of gels eliminated aberrant migration patterns.