Characterization of a New Radiation-Sensitive Mutant, Escherichia coli K-12 radC102

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Abstract
A new radiation-sensitive mutant, radC, was isolated. The radC gene is located at 81.0 min on the E. coli K-12 linkage map. The radC mutation sensitized cells to UV radiation, but unlike most DNA repair mutations, sensitization to X-rays was observed only for rich medium-grown cells. For cells grown in rich medium, the radC mutant was normal for .gamma.-radiation mutagenesis, but showed less UV-radiation mutagenesis than the wild-type strain; it showed normal amounts of X- and UV-radiation-induced DNA degradation, and it was .apprx. 60% deficient in recombination ability. The radC strain was normal for host cell reactivation of .gamma.- and UV-irradiated bacteriophage .lambda.; the radC mutation did not sensitize a recA strain, but did sensitize a radA and a polA strain to X and UV radiation and a uvrA strain to UV radiation. The radC gene product may play a role in the growth medium-dependent, recA gene-dependent repair of DNA single-strand breaks after X irradiation, and in post-replication repair after UV irradiation.

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