Amino acid sequence of the variable regions of heavy chains from two idiotypically cross-reactive human IgM anti-.gamma.-globulins of the Wa group
- 29 September 1981
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 20 (20) , 5822-5830
- https://doi.org/10.1021/bi00523a027
Abstract
Amino acid sequences of the variable regions of the heavy chains derived from 2 idiotypically related human monoclonal rheumatoid factors are reported. The sequences were obtained through automated Edman degradations of the intact, pyroglutamate aminopeptidase cleaved heavy chains and peptides produced from digestion of the pentameric IgM with CNBr. The peptides generated from the CNBr reaction were further digested by trypsin and the Staphylococcus aureus V8 protease. Comparisons of these sequences and those of the light chains from these molecules suggest that the idiotypic determinant(s) in this system may reside in the framework portions of these molecules or in their J segments.This publication has 6 references indexed in Scilit:
- Nucleotide sequence of immunoglobulin heavy chain joining segments between translocated VH and mu constant regions genes.Proceedings of the National Academy of Sciences, 1980
- Amino acid sequence of the variable region of a human mu chain: location of a possible JH segment.Proceedings of the National Academy of Sciences, 1980
- Amino acid sequence of homogeneous antibodies to dextran and DNA rearrangments in heavy chain V-region gene segmentsNature, 1980
- Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.Proceedings of the National Academy of Sciences, 1979
- Sequences at the somatic recombination sites of immunoglobulin light-chain genesNature, 1979
- Sequences of mouse immunoglobulin light chain genes before and after somatic changesCell, 1978