Analysis of d-ethambutol by circular dichroism of its copper chelates.

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Abstract
The antituberculostatic agent ethambutol, possessing a little molar specific rotation, was converted to several metal chelated derivatives. Among them, the Cu chelate showed a large ellipticity value in its circular dichroism (CD) spectrum. A quantitative analysis of ethambutol by means of the Cu chelation followed by CD spectroscopic measurements were investigated. Addition of 0.1 ml of 0.3% ethanolic solution of ethambutol to 2.9 ml of 0.01% ethanolic solution of CuCl2 ready to form Cu chelated ethambutol which gave CD ellipticity value 4.6 .+-. 0.3 in its CD spectrum. The present method is one of the convenient procedures to introduce a chromophoric nature to an optically active amino alcohol for a CD measurement.