Chemiluminescence determination of penicillamine via flow injection applying a Quinine–cerium(IV) system

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Abstract
A rapid flow injection method with chemiluminescence detection is described for the determination of penicillamine. The method is based on the chemiluminescence reaction of thiol-containing drugs with cerium(IV) in sulfuric acid medium. Quinine is suggested as a fluorescer for the energy-transferred excitation process. The optimum conditions for chemiluminescence emission were investigated. With peak height as a quantitative parameter, penicillamine can be determined over the concentration range 2–200 µmol l–1 with a detection limit of 15 pmol of original thiol. The method was applied to the determination of penicillamine in a pharmaceutical tablet preparation.