The mechanism of nicotinuric acid synthesis

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Abstract
An enzyme preparation has been obtained from acetone-dried ox-liver mitochondria which synthesized nicotinuric acid from nicotinic acid, glycine, adenosine triphosphate and coenzyme A (CoA). It also formed nicotinoylhydroxamic acid, which was identified chromatographically, when glycine was replaced by high concentrations of hydroxylamine. Both reactions occurred without added CoA but showed about 50% stimulation in the presence of CoA (40 units/ml). With a Dowex-1 treated enzymes solution, the syntheses in the absence of added CoA were decreased several fold, and in both cases a 260-270% increase in activity was found when CoA (40 units/ml) was added.