One-Step Enzyme-Linked Immunosorbent Assay (ELISA) for Plasma Fatty Acid-Binding Protein
Open Access
- 1 May 1997
- journal article
- research article
- Published by SAGE Publications in Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
- Vol. 34 (3) , 263-268
- https://doi.org/10.1177/000456329703400307
Abstract
To allow a more rapid determination of heart-type fatty acid-binding protein (FABP) concentration in plasma a direct non-competitive (sandwich-type) ELISA was developed which uses high-affinity monoclonal antibodies to FABP. Total performance time of the one-step immunoassay is 45min. The standard curve was linear between 0·2–6μg/L, and the within-run and between-run coefficients of variations were below 6 and 11%, respectively. The serum FABP concentration measured in 79 healthy individuals was 1·6 (0·8) [mean (SD), range 0·3–5·0] μg/L. The assay can be used for rapid plasma or serum FABP measurement in the early diagnosis of acute myocardial infarction.Keywords
This publication has 16 references indexed in Scilit:
- Cardiac Troponin T Release into Plasma after Acute Myocardial Infarction: Only Fractional Recovery Compared with EnzymesAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1996
- Development of a sandwich enzyme-linked immunosorbent assay for the determination of human heart type fatty acid-binding protein in plasma and urine by using two different monoclonal antibodies specific for human heart fatty acid-binding proteinJournal of Immunological Methods, 1995
- Discrimination Between Myocardial and Skeletal Muscle Injury by Assessment of the Plasma Ratio of Myoglobin Over Fatty Acid–Binding ProteinCirculation, 1995
- Fatty-acid-binding protein as a plasma marker for the estimation of myocardial infarct size in humans.Heart, 1994
- Human heart-type cytoplasmic fatty acid-binding protein in serum and urine during hyperacute myocardial infarctionInternational Journal of Cardiology, 1993
- Biochemical markers of myocardial injury. Is MB creatine kinase the choice for the 1990s?Circulation, 1993
- Release of heart fatty acid-binding protein into plasma after acute myocardial infarction in manMolecular and Cellular Biochemistry, 1992
- Serum and urinary human heart fatty acid-binding protein in acute myocardial infarctionClinical Biochemistry, 1991
- Rabbit heart fatty acid-binding protein. Isolation, characterization, and application of a monoclonal antibody.Circulation Research, 1989
- Release of fatty acid-binding protein from isolated rat heart subjected to ischemia and reperfusion or to the calcium paradoxBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1988