DNA repair dependent NAD+ metabolism is impaired in cells from patients with Fanconi's anemia.

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Abstract
In vitro cultivated fibroblasts derived either from patients with Fanconi's anemia (FA) or from healthy probands were analyzed for their DNA repair‐dependent NAD+ metabolism. No difference in NAD+ pools was found. NAD+ consumption after cell damage by u.v. irradiation was, however, significantly reduced in FA cells. Several FA cell lines had a lowered ability to transfer ADP‐ribose to acid‐precipitable material. Additionally, a decreased activity of NAD: protein ADP‐ribosyltransferase was found for three FA cell lines. Our data indicate, that FA is accompanied by a defective NAD+ metabolism during DNA repair.