Effect of ionic environment on metabolism and structure of rat kidney slices

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Abstract
The ionic composition of isoosmotic incubation media exerts a profound effect on the net uptake of glucose, on metabolism of glucose to CO2, and on net water content in rat kidney slices. Substitution of the principal cation, sodium, of the standard incubation medium with potassium, choline, lithium, or guanidine results in characteristic alterations. Sodium is not essential for glucose penetration into the slices. Substitution of the principal anion, chloride, has relatively little effect on metabolism and net water content. A reduction in net glucose uptake and metabolism to CO2 is seen in the high-potassium and choline media. In the potassium medium, there is a striking uptake of water and histological examination reveals marked disruption of tubular cells. In the choline medium, as in the standard medium, there is a net water loss and cellular architecture is well preserved. Under anaerobic conditions, the marked water uptake is partially inhibited in the choline medium.

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