Inhibition of secondary anti‐hapten responses with the hapten conjugated to type 3 pneumococcal polysaccharide
- 1 October 1972
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 2 (5) , 460-467
- https://doi.org/10.1002/eji.1830020516
Abstract
An injection of 2, 4‐dinitrophenyl (DNP)‐lysine conjugated to type 3 pneumococcal polysaccharide (DNP‐lysine‐SIII) markedly reduced the secondary IgG anti‐DNP antibody response of irradiated mice injected with primed cells and the homologous dinitrophenylatcd foreign protein used for priming. Serum antibody titers against the foreign protein, hemocyanin, were not affected and the injection of DNP‐lysine‐SIII could be delayed until the 3rd day, but not the 4th day, after injection of the primed cells and homologous antigen. In mice in which anti‐DNP antibody production was reduced by DNP‐lysine‐SIII, antibody isoelectric spectral analysis of residual antibody suggested that clones of antibody‐producing cells were eliminated entirely or escaped unaltered. Purified SIII is a persisting antigen which readily induces macroglobulin responses in mice and does not seem to stimulate T cells. The results suggest that antigenic determinants on such antigens gain access to B cells and suppress IgC antibody production.Keywords
This publication has 40 references indexed in Scilit:
- Interaction between lymphocytes in immune responsesCellular Immunology, 1971
- Evidence for thymus-independent humoral antibody production in mice against polyvinylpyrrolidone and E. coli lipopolysaccharideCellular Immunology, 1971
- THE EFFECT OF ANTILYMPHOCYTIC ANTIBODY ON THE PRIMARY IMMUNE RESPONSE OF MICE TO SHEEP ERYTHROCYTES, BOVINE SERUM ALBUMIN, AND TYPE III PNEUMOCOCCUS POLYSACCHARIDETransplantation, 1971
- Antibody isoelectric spectra. Analysis of the heterogeneity of antibody molecules in serum by isoelectric focusing in gel and specific detection with haptenEuropean Journal of Immunology, 1971
- A Simple Hemolytic Plaque Technique for the Enumeration of Anti-Hapten Antibody Forming CellsInternational Archives of Allergy and Immunology, 1971
- DETERMINATION OF ANTIBODY CLASS IN A SYSTEM OF COOPERATING ANTIGENIC DETERMINANTSThe Journal of Experimental Medicine, 1970
- CELL TO CELL INTERACTION IN THE IMMUNE RESPONSEThe Journal of Experimental Medicine, 1970
- POLYSACCHARIDE IN DELAYED HYPERSENSITIVITYThe Journal of Experimental Medicine, 1970
- Glutaraldehyde, cyanuric choloride and tetraazotiexe O-dianisidine as coupling reagents in the passive hemagglutination testImmunochemistry, 1969
- Transplant DonorsBMJ, 1969