Chromatin conformational changes accompany transcriptional activation of a glucose-repressed gene in Saccharomyces cerevisiae.

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Abstract
The kinetics of DNase I digestion of the 2 alcohol dehydrogenase (ADH) genes within yeast nuclei were analyzed. In yeast grown on glucose the constitutively transcribed ADC1 gene is much more sensitive to DNase I digestion than is the repressible ADR2 gene. In yeast grown on ethanol, both genes are transcribed and both exhibit the same sensitivity to DNase I attack. We have also found and mapped DNase I hypersensitive sites near the 5'' ends of constitutive and repressible ADH genes. These sites are well correlated with the position at which transcription is initiated.