The interaction of ribosomal protein L16 and its fragments with tRNA
- 21 March 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 153 (2) , 267-269
- https://doi.org/10.1016/0014-5793(83)80621-8
Abstract
Two large proteolytic fragments of Escherichia coli 50 S ribosomal subunit protein L16 were generated by limited hydrolysis with chymotrypsin (missing 9 N-terminal amino acids) and trypsin (missing 16 N-terminal amino acids). It was found that while intact L16 and its chymotryptic fragment both interact with tRNA (Kd = 5.4 x 10−7 M), the tryptic fragment does not. These results are interpreted in terms of possible significance of the residues 10–16 in the peptidyl transferase activityKeywords
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