Examination of Ligand-Dependent Coactivator Recruitment by Peroxisome Proliferator-Activated Receptor-(PPAR)
Open Access
- 27 June 2006
- journal article
- research article
- Published by Hindawi Limited in PPAR Research
- Vol. 2006, 1-9
- https://doi.org/10.1155/ppar/2006/69612
Abstract
The ligand-dependent recruitment of coactivators to peroxisome proliferator-activated receptor- (PPAR) was examined. PPAR-binding protein (PBP), PPAR coactivator-1 (PGC-1), steroid receptor coactivator-1 (SRC-1), and CBP/p300-interacting transactivator with ED-rich tail 2 (CITED2) affected PPAR activity in the presence of Wy-14,643. The effects on PPAR activity in light of increased or decreased expression of these coactivators were qualitatively different depending on the ligand examined. Diminished expression of PGC-1, SRC-1, or PBP by RNAi plasmids affected natural or synthetic agonist activity whereas only Wy-14,643 was affected by decreased PGC-1. The interaction of PPAR with an LXXLL-containing peptide library showed ligand-specific patterns, indicative of differences in conformational change. The association of coactivators to PPAR occurs predominantly via the carboxyl-terminus and mutating 456LHPLL to 456LHPAA resulted in a dominant-negative construct. This research confirms that coactivator recruitment to PPAR is ligand-dependent and that selective receptor modulators (SRMs) of this important protein are likely.Keywords
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