The isolation of cloned cDNA sequen which are differentially expressed in human lymphocytes and fibroblasts

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Abstract
Poly(A) + RNA populations derived from normal lymphocytes and fibroblasts have been compared by hybridising each RNA to cDNA derived from the other RNA population. This indicated that approximately 75% of the sequences were common to both, and that these were present at different concentrations in the two cell types. The two RNA populations were further compared by hybridising them to a cDNA recombinant library derived from lymphocyte poly(A) + RNA. This allowed the identification of clones containing sequences which are abundant in lymphocyte poly(A) + RNA but absent or rare in fibroblast poly(A) + RNA. A direct estimation of the abundance of five of these sequences in lymphocyte cDNA demonstrated that clones can be detected by such a procedure if they represent 0.2% or greater of the original cDNA population.