Contact sensitivity in the pig.

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  • 1 December 1973
    • journal article
    • Vol. 25  (6) , 995-1009
Abstract
Following the application of a sensitizing dose of DNFB the afferent lymph draining the site becomes yellow due to the presence of dinitrophenylated proteins. Intralymphatic perfusion of such afferent lymph either whole or cell-free into recipients led to the development of contact sensitivity to DNFB in these animals. All of the OD360, due to the DNP group, in the afferent lymph was precipitable with trichloracetic acid and at least 90 per cent non-dialysable. Preliminary data indicate that the DNP is conjugated to eight or more proteins; however, the chemical nature of the carrier(s) responsible for the induction of contact sensitivity and whether it is derived from interstitial fluid or skin are unknown.