Unique V kappa group associated with two mouse L chain genetic markers.

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Abstract
The C.C58 and C.AKR congeneic strains of mice differ from BALB/c at loci on chromosome 6 which govern .kappa. light chain variable region (V.kappa.) polymorphisms and the Lyt-2 and Lyt-3 alloantigens. Amino acid sequence analysis of light chains of myelomas induced in these strains revealed 1 light chain, C.C58 M75, that had an NH2-terminal serine and differed sufficiently from published V.kappa. sequences to define a new V.kappa. group, V.kappa.(Ser), apparently not expressed by BALB/c mice. Peptide map analysis indicated that the M75 light chain contained the IB-peptide marker, a V.kappa. polymorphism expressed by C.C58 but not BALB/c mice, which is determined by the IgK-Trpa allele present on chromosome 6. This same light chain was found by isoelectric focussing to correspond to IgK-EfIa, another V.kappa. genetic marker of C.C58 and C.AKR. Isoelectric focussing of approximately 200 C.C58 and C.AKR myeloma light chains revealed 3 additional C.C58 and 4 C.AKR light chains that corresponded to IgK-EfIa-specific light chains. All 3 additional C.C58 light chains belonged to the V.kappa.(Ser) group and contained the IB-peptide marker. The differences in V.kappa. repertoires represented by the IB-peptide and IgK-Efla markers and controlled by genes on chromosome 6 appear to reflect expression (or failure of expression) of a distinct group of V.kappa. regions.